Abstract

Objective: Our aim was to explore the effects of short bowel syndrome (SBS) on CD14 mRNA expression in the liver and examine ultrastructural and biochemical changes in a rat model of total parenteral nutrition (TPN) independent SBS.

Material and Methods: Male Wistar Albino rats, weighing 180 to 220 g (n=37) were randomly divided into two groups as sham operated (n=16) and short bowel syndrome (n=21). Division and re-anastomosis of the bowel 15 cm proximal to the ileocecal junction was performed in sham operated animals. 75% of the small intestine was resected in the short bowel syndrome group. All animals were pair-fed. CD14 mRNA expression was evaluated semiquantitatively via RTPCR. Concomitantly, liver function test were performed and ultrastructural changes in the liver were evaluated.

Results: There was no statistical significant difference regarding liver function tests. There were no significant differences for inflammation, fibrosis and steatosis on histological examination of liver. Cholestasis was more prominent in the SBS group (p<0.05). There were marked ultrastructural changes on electron microscopy. Microvilli canaliculus length, presence of luminal granular biliary material and luminal length of canaliculus were increased in the SBS group (p<0.05). CD14 expression was significantly increased in the liver in the SBS group (p<0.05). The correlation between the ultrastructural changes and CD14 mRNA expression was also prominent (p<0.05)

Conclusion: Cholestasis was demonstrated ultrastructurally in this rat model of TPN independent SBS. Increased expression of CD14 mRNA in the SBS group supports that these changes might occur because of portal venous endotoxemia

Keywords: Cholestasis, Endotoxemia, Hepatic failure, PCR, Short bowel syndrome

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How to cite

1.
Göllü G, Şenyücel M, Akyürek N, Gönül İ, Özkul A, Gökçora H, et al. Ultrastructural Changes and CD14 Expression in the Liver in the Short Bowel Syndrome. Turk J Pediatr Dis [Internet]. 2018 Aug. 1 [cited 2025 May 25];12(2):79-85. Available from: https://turkjpediatrdis.org/article/view/519